Primer designing tool - National Center for Biotechnology …
Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type. Help Entrez query (optional) Help …
National Center for Biotechnology Information
Try "re-search for specific primers" feature on Primer-BLAST result page. When only non-specific primers are found, the result page offers the option to re-search for specific primers.
Basic Local Alignment Search Tool - BLAST
Jul 21, 2025 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence …
Primer-BLAST Archives - NCBI Insights
To find primers for a group of related sequences, Primer-BLAST aligns the longest sequence to the rest to find common regions. It uses these to limit the locations of primers. The longest …
Primer-Blast results - National Center for Biotechnology Information
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.
CREPE (CREate Primers and Evaluate): a computational tool for …
Mar 28, 2025 · Its final output summarizes the lead forward and reverse primer pair for each target site, a measure of the likelihood of binding to off-targets, and additional information to …
Primer-BLAST: a tool to design target-specific primers for
Jun 18, 2012 · We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers. Primer-BLAST offers flexible options to adjust the …
Electronic-PCR (e-PCR) is retiring, use Primer-BLAST instead
Jun 28, 2017 · The good news is that an existing tool, Primer-BLAST, fills in nicely for the functions of both Forward and Reverse e-PCR, and has the additional benefit of de novo …
Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing
Primer choice influences the estimated microbial composition. A set of different 16S rRNA gene sequencing primer pairs covering one, two, or three of the variable regions V1 to V9 is …
5-alpha-reductase type I (SRD5A1) is up-regulated in non-small …
Following RNA extraction and cDNA synthesis, qRT-PCR was performed using two different primer sets specific for SRD5A1. In parallel, the expression levels for the housekeeping gene …